ABO anticorps

N° du produit ABIN6252832

L’anticorps Souris Monoclonal anti-ABO a été validé pour IF, Func et IHC (p). Il convient pour détecter ABO dans des échantillons de Humain.

Aperçu rapide pour ABO anticorps (ABIN6252832)

Antigène: ABO (Histo-blood group A transferase (ABO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABO est non-conjugé

Application: Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HE-14

Détail du produit anti-ABO anticorps

Purification: PEG precipitation

Immunogène: A mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Functional Activity-Erythrocyte Agglutination

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Optimal dilution of the ABO antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur ABO

Antigène: ABO (Histo-blood group A transferase (ABO))

Autre désignation: ABO

Sujet: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 mAb was used.

ID gène: 28

UniProt: P16442